Rapid communication: three new allelic sequences at the ovine MHC class II DQA1 locus.

摘要: DNA poly-merase (Qiagen GmbH, Hilden, Germany) (consistingof initial denaturation at 94°C for 90 s, followed by 32cycles of 30 s 94°C, 60°C, and 72°C,with a final extension 5 min 72°C) were clonedinto pGEM T-Easy vector (Promega, Madison, WI) andboth strands sequenced the Se-quencing Facility University Waikato, NewZealand usingM13 primers.At leastthree independentPCR amplifications each sequence carried outto confirm identity, only identical se-quences from least three independent amplificationswere analyzed. Of number sequences obtained,three showed differences those pre-viously published.