High-performance liquid chromatographic determination of leucine-enkephalin-like peptide in rat brain by pre-column fluorescence derivatization involving formylation followed by reaction with 1,2-diamino-4,5-dimethoxybenzene.

作者: Masaaki Kai , Junichi Ishida , Yosuke Ohkura

DOI: 10.1016/S0378-4347(00)83162-X

关键词: FluorescenceDerivatizationFormylationOpioid peptideChemistryPeptideChromatographyChloroformFluorescence spectroscopyResidue (chemistry)

摘要: … with 3 ml each of water and methanol. After … ml of water, 2 ml of dichloromethane to remove strongly hydrophobic substances, 1 ml of water, 3 ml of 0.1 M hydrochloric acid, 1 ml of water, …

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