Recovery of plants from cryopreserved embryogenic cell suspensions of Pinus caribaea.
作者： Eric Laine , Pascale Bade , Alain David
DOI: 10.1007/BF00235085
关键词: Cell culture 、 Cell 、 Biology 、 Botany 、 Pinus <genus> 、 Liquid nitrogen 、 Embryo 、 Cryoprotectant 、 Sucrose 、 Cryopreservation
摘要: Embryogenic cell suspension cultures of Pinus caribaea var. hondurensis have been cryopreserved in liquid nitrogen for up to four months, using sucrose and dimethylsulfoxide as cryoprotectants. Post-thaw growth was obtained after a short lag phase. Removal the remaining around cells filter disc favoured subsequent regrowth cells. These reestablished maintained an embryogenic potential similar non-frozen cultures. The embryos produced were able regenerate into plants, which are now growing greenhouse.
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