Improved in vitro angiogenic behavior on anodized titanium dioxide nanotubes.

摘要: Neovascularization over dental implants is an imperative requisite to achieve successful osseointegration onto implanted materials. The aim of this study was investigate the effects on in vitro angiogenesis anodized 70 nm diameter TiO2 nanotubes (NTs) Ti6Al4V alloy synthesized and disinfected by means a novel, facile, antibacterial cost-effective method using super oxidized water (SOW). We also evaluated role surface roughness chemical composition materials angiogenesis. commercially pure Ti were solution constituted SOW fluoride as electrolyte. An acid-etched compare effect micro-surface roughness. Mirror-polished used control. Morphology, roughness, chemistry wettability assessed field emission scanning electron microscopy (FE-SEM), transmission microscopy, atomic force energy dispersive X-ray spectroscopy (EDX) professional digital camera. Bovine coronary artery endothelial cells (BCAECs) seeded experimental surfaces for several incubation times. Cellular adhesion, proliferation monolayer formation SEM. BCAEC viability, actin stress fibers vinculin cellular organization, well angiogenic receptors vascular growth factor 2 (VEGFR2) nitric oxide synthase (eNOS) measured fluorescence microscopy. anodization process significantly increased thickness coating. EDX analysis demonstrated oxygen (O) decreased carbon (C) content NTs both Endothelial behavior solidly supported improved (without significant differences between alloy), showing that proliferation, arrangement with expression development evidently stimulated nanostructured surface, leading activation VEGFR2 eNOS Ti6Al4V-NTs compared control alloy. Although rougher promoted BCAECs viability filopodia poor. results suggest manufactured cleaned promotes activity, which may improve vivo supporting faster clinical process.