C2C12 myoblastoma cell differentiation and proliferation is stimulated by androgens and associated with a modulation of myostatin and Pax7 expression

摘要: Androgens are modulators of skeletal muscle adaptation and regeneration processes. The control satellite cell activity is a key mechanism during this process. In study, we analyzed the ability dihydrotestosterone (DHT) anabolic steroids to induce modulate differentiation C2C12 myoblastoma cells toward myotubes. were dose-dependently treated with DHT steroids. time-dependent effects on measured correlated expression genes involved in regulation activity. distribution within cycle was by flow cytometry creatine kinase (CK) Gene using quantitative real-time PCR confocal microscopy. treatment resulted stimulation proliferation CK antiandrogen flutamide able antagonize effect. androgen receptor, SOX8, SOX9, Delta, Notch, myostatin, paired box gene7 (Pax7) modulated androgens. strong myostatin not only undifferentiated but also could be antagonized flutamide. Pax7 detectable early after DHT. Our results demonstrate that mechanisms stimulate cells, accelerate process differentiation, increase differentiated cells. findings may have implications for muscular diseases improvement doping analytical methods.