pH-Controlled Diffusion of Proteins with Different pI Values Across a Nanochannel on a Chip

摘要: Electrostatic interactions dominate the diffusion of proteins in a nanochannel, which was measured and modeled for pH values above below isoelectric points three lectin proteins. Maximal coefficients were obtained when neutral at their pI. This pH-controlled transport led to separation biomolecules across nanochannel. A pI shift observed, due reversible adsorption on walls, affects