Portable device for continuous fractionated blood sampling and continuous ex vivo blood glucose monitoring

摘要: The objective of the study was to develop and evaluate a portable device for continuous fractionated blood sampling ex vivo monitoring glucose. inner lumen double catheter (18 gauge x 45 mm) placed in peripheral vein perfused with heparin solution (1.4 U min-1). outer used collect heparinized into 48 vacuum tubes at programmable sample volumes time intervals (0.2-2 ml 2.5-30 min). A sensor flow chamber an internal volume 1 mm3 incorporating miniaturized thin-film amperometric glucose line monitoring. Blood plasma insulin were measured during frequently sampled intravenous tolerance test (250 mg kg-1) subsequent oral (150 g) over 6 h eight healthy volunteers (BMI 24.5 +/- 3.2 kg m-2). Additionally, four experiments on-line using sensors. overall correlation coefficients whole between manually drawn samples 0.73 0.87, respectively (p < 0.001). exhibited linear measuring range 25 mmol-1 concentration 95% response times less than 30 s. Sensor readings laboratory analyser results measurement correlated 0.93 0.98 In summary, ambulatory subjects is possible device.