In vitro translation of Harvey murine sarcoma virus RNA.

摘要: The viral RNA of the Harvey strain murine sarcoma virus (Ha-SV), which does not encode for any known structural polypeptides, has been translated in a nuclease-digested, cell-free system. major protein product vitro translation reaction molecular weight 21,000 and is initiated faithfully with [35S]formylmethionine from formyl-[35S]methionyl-tRNAFMET. This polypeptide clearly distinct Moloney type C helper used to pseudotype Ha-SV. intensity 21,000-dalton on gels correlates well concentration Ha-SV different preparations. These experiments indicate that marker now available first time. possibility this rat portion genome discussed.