Expression of extracellular superoxide dismutase by human cell lines.

摘要: Extracellular superoxide dismutase (EC-SOD) is the major SOD isoenzyme in extracellular fluids, but occurs also tissues. The sites and characteristics of synthesis enzyme are unknown. occurrence EC-SOD cultures a large panel human cell lines was assayed by means an e.l.i.s.a. Unlike situation for intracellular isoenzymes CuZn-SOD Mn-SOD, expression only few types. None ten investigated suspension-growing produced EC-SOD. Among normal diploid anchorage-dependent lines, found all 25 fibroblast two glia-cell not six endothelial-cell epithelial-cell or amnion-derived lines. neoplastic 13 out 29. secreted into culture medium expressing enzyme. rate varied nearly 100-fold among remained essentially constant individual during long-term culture. In nine cases, high-heparin-affinity C type. It suggested that tissue well-dispersed types, such as fibroblasts glia cells, to diffuse subsequently around reversibly bind heparan sulphate proteoglycan ligands glycocalyx surface most types interstitial matrix.