Electrically conductive magnetite particles enhance the kinetics and steer the composition of anaerobic TCE-dechlorinating cultures

摘要: Abstract The interaction of anaerobic dechlorinating cultures with soil and aquifer geochemical components is largely unknown, although this has potentially a major impact on the bioremediation chlorinated solvent-contaminated sites. In study, we found that addition magnetite (Fe 3 O 4 ) – end-product Fe(III)-reduction by dissimilatory iron reducing bacteria to enhances kinetics trichloroethene dechlorination up 1.5-times, compared unamended controls. Specifically, low concentration (approx. 10 mg/L as total Fe) small size particles (200 nm-filtered) resulted in greater stimulatory effect higher 300 mg/L unfiltered particles. Notably, Desulforomonas spp. were substantially enriched microcosms supplemented magnetite, whereas Dehalococcoides mccartyi was be markedly inhibited or outcompeted. Multiple lines evidence, including direct visualization microbial cells via Confocal Laser Scanning Microscopy (CLSM), suggest electrically conductive promoted establishment cooperative metabolism, based interspecies electron transfer, between non-dechlorinating microorganisms.