作者: Yngve Melander , Karl Georg Wingstrand
DOI: 10.3109/10520295309105236
关键词: Hydrolysis 、 Hydrochloric acid 、 Biochemistry 、 Acetic acid 、 Feulgen stain 、 H&E stain 、 Staining 、 Stain 、 Chemistry 、 Phloxine 、 Chromatography
摘要: The chromic hematoxylin of Gomori (1941) can be used as an excellent chromosome stain after hydrolysis the tissue in warm 1–N hydrochloric acid. must accurately timed for different material case Feulgen reaction. staining sections performed at room temperature and requires about 15 minutes. For pieces whole preparations, it is recommended to 60°C. 40 Sections stained differentiated 1% acid alcohol one minute counterstained with phloxine according Gomori's formula. Whole preparations or 45% acetic half hour more. Tissue may, staining, squashed examined acid, but also made permanent. blue-black very selective has advantage giving high contrast, nonfading, insoluble water other common reagents. ...