Differential stain for the human and animal anterior hypophysis.

摘要: A staining method is described for human and animal pituitary glands which based on a combination of modification the phosphomolybdic acid, orange G stain Berblinger Burgdorf's (1935) method, with an iron hematoxylin nuclei periodic-acid-Schiff routine Hotchkiss McManus. The cytochemical part procedure demonstrates both muco-protein precursors gonadotropins and, it thought, these hormones themselves. By comparison other methods, finer differences in cytology cyanophils can be appreciated. Quantitative cell counts are readily performed large numbers cells, appearing by Mallory histological methods to chromophobes, found belong cyanophil series. Counts done older therefore, considered inaccurate misleading.