Histochemical and Autoradiographic Studies on the Effects of Aging on the Mucopolysaccharides of the Periosteum

摘要: An autoradiographic study was made using S35-sulfate for the localization, distribution, and variation in mucopolysaccharide content of femoral periosteum rats from birth to old age. The mucopolysaccharides were also studied histochemically, toluidine blue O, Rinehart Abu'l-Haj's colloidal iron method, periodic acid-Schiff reaction, before after hyaluronidase treatment. Autoradiograms revealed uptake S35 particularly vicinity preosseous zone adjacent osteoblasts. This labelling highest at period rapid bone growth. With increasing age, became progressively less. showed γ-metachromatic staining all ages treatment with blue. Active osteoblasts mostly orthochromatic, however, β-metachromasia exhibited a later Abundant amounts intra- extracellular both acid neutral type demonstrated periosteum. γ-metachromasia show presence sulfated mucopolysaccharides, which chondroitin sulfate predominates zone. Since is high active osteoblasts, inability demonstrate metachromasia may indicate either that liberated as fast it being produced, or be present within cells precursor form not detectable by histochemical methods.