Human Lymphotoxin Obtained from Established Lymphoid Lines: Purification Characteristics and Inhibition by Anti-Immunoglobulin

摘要: Human lymphotoxin obtained from established lymphoid cell lines (HLT-LCL) was studied in detail and purified using ammonium sulfate fractionation, gel filtration, ion-exchange column chromatography, polyacrylamide electrophoresis. HLT-LCL is stable at 20°C for over 1 year, 4°C 3 weeks, 37°C week. showed a decrease activity pH 2.0 3.0, but range of 5.0–11.0 HLT-LCL, which contained fetal calf serum, 50-fold. serum-free culture medium yielded greater purity, although complete purification not achieved. Antiserum to neutralized the cytotoxic HLT induced by PHA (HLT-PHA), PPD (HLT-PPD). human IgG, λ-type L chain, Fc fragment partially significantly inhibited activity. These data, together with previous reports, indicate that molecule immunologically similar HLT-PHA HLT-PPD suggest there immunologic cross-reactivity IgG globulin.