Monoclonal antibodies to a MUC 4 peptide react with lung cancer.

摘要: The cDNA of mucin 4 (MUC 4) has been cloned from human tracheo-bronchial library and MUG was demonstrated to be expressed in normal tracheobronchial colonic mucosa. However, as there are no antibodies available, is little information on the distribution MUC malignant tissues. A 22 amino-acid peptide (M4.22), derived sequence synthesized used immunise mice 2 monoclonal were produced characterized by ELISA, immunoperoxidase staining flow cytometry. By ELISA two anti-MUG mAbs reacted with immunizing but not peptides other mucins. native colon a lesser extent stomach, lung. contrast, most strongly (similar 70%) lung cancers. Using six-mer overlapping minimum number amino acids epitopes detected TPL (M4.171) PLPV (M4.275). molecular mass (180 kDa) Western blotting. anti-MUC non-large cell cancers found positive compared tissues which negative. expression decreased cancer. These findings could have diagnostic therapeutic implication for