DNA origami nanopores: an emerging tool in biomedicine

摘要: Natural systems have developed a precise and intricate machinery to preserve life. The com­ plex mechanisms that living organisms employ control the transport of ions mole­ cules across their lipid membranes represent remarkable example. Membrane is indisputable importance, as it ultimately implicated in such important functions energy production protein synthesis. Many pathways are mediated by pres­ ence small holes (known nanopores biotechnology) based on active or passive proteins. Since organ­ isms so efficiently utilize these protein­based passage biomolecules, why not them obtain devices with potential interest biotechnology biomedicine? A key achievement was detection single molecules utilizing nano­ pores. Two decades ago, Kasianowicz et al. [1] were able detect DNA RNA mol­ ecules for first time using natural pore (a­hemolysin) inserted into an artificial membrane means resistive pulse technique [2]. idea easy elegant: indi­ vidual pass through nanopore lead­ ing changes ionic current characteristic translocating molecule. a­hemolysin (a Staphylococcus aureus pore­ forming protein) has been far most employed biological due its dimensions, stability commercial availability. main advantage pores perfectly defined structure, which guar­ antees reproducibility measurements possibility introducing different chemi­ cal groups mutating sequence pore. This can be used increase