DNA-Tile Structures Induce Ionic Currents through Lipid Membranes

摘要: Self-assembled DNA nanostructures have been used to create man-made transmembrane channels in lipid bilayers. Here, we present a DNA-tile structure with nominal subnanometer channel and cholesterol-tags for membrane anchoring. With an outer diameter of 5 nm molecular weight 45 kDa, the dimensions our synthetic nanostructure are comparable biological ion channels. Because its simple design, self-assembles within minute, making creation scalable applications biology. Ionic current recordings demonstrate that tile structures enable conduction through bilayers show gating voltage-switching behavior. By demonstrating design DNA-based openings much smaller than archetypical six-helix bundle, work showcases their versatility inspired by rich diversity natural components.