Changes in pulmonary phospholipid biosynthetic enzymes after nitrogen dioxide exposure.

摘要: Abstract The activities of phospholipid synthesizing enzymes and marker were measured in homogenates subcellular fractions lungs livers rats 2 days after exposure to 42 ppm nitrogen dioxide (NO ) for 5 hr. DNA content NO -exposed increased 33% over air-exposed controls, while protein all was elevated about twofold. ratios wet dry weight remained unchanged. specific activity microsomal glycerol-3-phosphate acyltransferase 42% higher than phosphatidate phosphohydrolase choline phosphotransferase 26 20% activity. enzyme NADPH cytochrome c reductase showed similar microsomes from or animals. Glycerolphosphate phosphatidyltransferase the mitochondrial succinate by 28 23% lungs. Total lung 80–180% No changes any parameters observed liver due exposure. These results are consistent with generally proliferative response cells toxic insult. large increase total biosynthesizing suggests that proliferating enriched these relative other cell types.