Adaptation of Rat Type II Pneumocytes to NO2: Effects of NO2Application Mode on Phosphatidylcholine Metabolism

摘要: Previous studies have shown that nitrogen dioxide (NO2) inhalation affects the extracellular surfactant as well structure and function of type II pneumocytes. Since in these there were great variabilities oxidant concentration, duration exposure, mode NO2 application, we evaluated influence application on phospholipid metabolism Rats exposed to identical body doses (720 ppm x h), which applied continuously (10 for 3 d), intermittently 8 h per day, 9 repeatedly d, 28 d rest, then 10 d). Immediately after cells isolated cell yield, vitality, phosphatidylcholine (PC) synthesis, secretion. Type pneumocyte yield from animals had been was significantly increased, whereas treated rats exhibited yields nonsignificantly enhanced. Vitality pneumocytes not affected by exposure modes. Continuous 720 resulted increased activity cytidine-5-diphosphate (CDP)-choline pathway. After continuous specific choline kinase, cytidine triphosphate (CTP):cholinephosphate cytidylyltransferase, uptake choline, pool sizes CDP-choline PC over those controls. Intermittent this dose also provoked an increase but less prominent than exposure. repeated synthesis parameters comparable control animals. Whereas obviously stimulated NO2, secretory reduced. reduced most, intermittent compared with The produced no differences. We conclude adapt atmospheres depending its at least secretion Further are necessary determine whether additional metabolic activities will atmospheres, if observations or represent effects generally due oxidants.