作者: Mark G. Nielsen , F.Rudolf Turner , Jeffrey A. Hutchens , Elizabeth C. Raff
DOI: 10.1016/S0960-9822(01)00150-6
关键词: Basal body 、 Genetics 、 Cilium 、 Biology 、 Microtubule 、 Tubulin 、 Centriole 、 Cell biology 、 Axoneme assembly 、 Sequence motif 、 Axoneme
摘要: Abstract Axonemes are ancient organelles that mediate motility of cilia and flagella in animals, plants, protists. The long evolutionary conservation axoneme architecture, a cylinder nine doublet microtubules surrounding central pair singlet microtubules, suggests all motile axonemes may share common assembly mechanisms. Consistent with this, α- β-tubulins utilized fall among the most conserved tubulin sequences [1, 2], contain sequence motif at same position carboxyl terminus [3]. Axoneme initiated from corresponding triplet basal body [4], but large macromolecular "central apparatus" includes associated structures [5] is specialization unique to axonemes. In Drosophila spermatogenesis, bodies utilize α-tubulin different [6–13]. β1 for centriole/basal body, β2 sperm tail axoneme. contains axoneme-specific motif, does not Here, we show "axoneme motif" specifies pair. can provide partial function cannot make [14]. Introducing into terminus, two amino acid change, conferred upon ability assemble 9 + 2 This finding explains through 1.5 billion years evolution.