Regulation of β-tubulin function and expression inDrosophila spermatogenesis

摘要: In this study we examined two aspects of β-tubulin function in Drosophila spermatogenesis: 1) structural requirements for assembly different categories microtubules and 2) regulatory production the correct tubulin protein level. normal spermatogenesis, testis-specific β2-tubulin isoform supports multiple microtubule functions. Our previous work showed that another isoform, β3, cannot support whereas a carboxyl-truncated form β2, β2ΔC, can at least to some extent provide all β2′s functions, save one: β2ΔC organization axonemal into supramolecular architecture axoneme. Here, test whether β2 carboxyl sequences rescue functional failure β3 constructed gene encoding chimeric protein, β3β2C, which region are replaced with those β2. Unlike either or β3β2C partial both their particular, mediate morphogenesis axoneme doublet tubule complex, including accessory attachment spokes linkers. However, our data also reveal β2-specific require features other than primary sequence isotype-defining variable regions, C terminus internal region. Tests fecundity males co-express Δ2 Δ3Δ2C there differential sperm motility male female reproductive tract. Since spermatogenesis sensitive pool size, mechanisms control levels germ cells. We found Δ2-tubulin mRNA accumulation synthesis dependent on dose, level expression is regulated by 3′ noncoding gene. show line differ from observed cultured animal somatic Finally, transgenic constructs consistent early cessation × chromosome spermatogenesis. © 1995 Wiley-Liss, Inc.