Production of elevated levels of dextransucrase by a mutant of Streptococcus mutans.

摘要: A mutant (S19) of Sreptococcus mutans strain 6715 which produces elevated levels dextransucrase (EC 2.4.1.5) was isolated. Soluble enzyme in culture supernatant solutions from S19 polymerized the glucosyl moiety sucrose into alcohol-insoluble and water-insoluble glucans at a rate three to six times greater than that parent strain. Washed-cell suspensions also contained increased amounts cell-associated enzyme. Adherence glass presence occurred twice 6715. The Km values for primer dextran were similar enzymes. Mutant should facilitate studies on mechanism adherence S. Mutans control production by this bacterium.