Long-term maternal separation differentially alters serum corticosterone levels and blood neutrophil activity in A/J and C57BL/6 mouse offspring.

摘要: Objectives: In this work, we searched for maternal separation effects on serum corticosterone levels and blood neutrophil activity in adult male A/J C57BL/6 mouse offspring. Methods: 40 mice were divided within each strain into two groups. Mice the group separated from their mothers (1 h/day) postnatal days 0–13. control left undisturbed. On day 45, was drawn all used to assess by flow cytometry radioimmunoassay. Results: The results showed that responded differently separation, but both cases, affected. strains, experienced lower than mice. relation kept together with mothers, of 72.7 36.36% submitted respectively. current findings increased after reaching adulthood. observed effects, although same direction, differed between Maternal percentage intensity phagocytosis mice, had no Furthermore, basal propidium iodide-labeled Staphylococcus aureus-induced oxidative burst did not affect Finally, phorbol myristate acetate-induced strains. Conclusion: These indicate early increases innate immunity, most likely modifying hypothalamus-pituitary-adrenal axis activity. This suggests is a good model stress which produces long-term neuroimmune changes whatever animal species used.