Long-range organization of a satellite DNA family flanking the beet cyst nematode resistance locus (Hs1) on chromosome-1 of B. patellaris and B. procumbens
作者： E. M. J. Salentijn , N. N. Sandal , R. Klein-Lankhorst , W. Lange , Th. S. M. De Bock
DOI: 10.1007/BF00225381
关键词: Heterodera schachtii 、 Ploidy 、 Gene 、 Nematode 、 Sequence analysis 、 Satellite DNA 、 Nucleic acid sequence 、 Biology 、 Genetics 、 Locus (genetics)
摘要: New members of a satellite DNA family (Sat 121), specific for wild beets the section Procumbentes genus Beta, were isolated. Sequence analysis showed that Sat-121 fall into two distinct classes. The organization in vicinity beet cyst nematode (Heterodera schachtii Schm.) resistance locus (Hs1) B. patellaris and procumbens was investigated by pulsed-field gel electrophoresis (PFGE) using from series resistant monosomic fragment additions, each containing an extra chromosome chromosome-1 (pat-1) vulgaris. In this way several clusters flanking Hs1 pat-1 identified. diploid introgressions (2n=18), which contain small segments (pro-1) vulgaris, only major detected near pro-1 locus.
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