The putative role of MALDI-MSI in the study of Membranous Nephropathy

摘要: Abstract Membranous Nephropathy (MN) is an immunocomplex mediated renal disease that represents one of the most frequent glomerulopathies worldwide. This glomerular can manifest as primary (idiopathic) or secondary and this distinction crucial when choosing appropriate course treatment. In cases, best strategy involves treating underlying disease, whereas in forms, identification confirmatory markers idiopathic etiology underlining process requested by clinicians. Among those currently reported, positivity to circulating antigens (PLA2R, IgG4 THSD7A) was demonstrated approximately 75% iMN patients, while 1 4 patients with still lack a putative diagnostic marker. Ultimately, discovery biomarkers help further stratify these two different forms glomerulopathy seems mandatory. Here, MALDI-MSI applied FFPE biopsies from histologically diagnosed MN (n = 20) order detect alterations their tissue proteome. able generate molecular signatures MN, particular signal (m/z 1459), identified Serine/threonine-protein kinase MRCK gamma, being over-expressed glomeruli respect MN. Furthermore, number signals could differentiate were positive PLA2R detected, well set 1094, 1116, 1381 1459) distinguish who negative both. These potentially represent future targets for stratification patients. article part Special Issue entitled: MALDI Imaging, edited Dr. Corinna Henkel Prof. Peter Hoffmann.