Limitations of MTT and CCK-8 assay for evaluation of graphene cytotoxicity

作者: Guozheng Jiao , Xiong He , Xin Li , Junqiang Qiu , Hongying Xu

DOI: 10.1039/C5RA08958A

关键词: Analytical chemistryToxicityIn vitro toxicologyCytotoxicityBromideBiophysicsChemistryViability assayLiver cellGrapheneHepg2 cells

摘要: … MTT and CCK-8 assay for determination of graphene cytotoxicity. MTT and CCK-8 assay are … of obtained data from MTT or CCK-8 assay in the presence of graphene related materials. …

参考文章(49)
Zhuang Liu, Joshua T. Robinson, Xiaoming Sun, Hongjie Dai, PEGylated Nanographene Oxide for Delivery of Water-Insoluble Cancer Drugs Journal of the American Chemical Society. ,vol. 130, pp. 10876- 10877 ,(2008) , 10.1021/JA803688X
Soo-Ryoon Ryoo, Young-Kwan Kim, Mi-Hee Kim, Dal-Hee Min, Behaviors of NIH-3T3 Fibroblasts on Graphene/Carbon Nanotubes: Proliferation, Focal Adhesion, and Gene Transfection Studies ACS Nano. ,vol. 4, pp. 6587- 6598 ,(2010) , 10.1021/NN1018279
Kun Yang, Wenhao Wu, Qingfeng Jing, Lizhong Zhu, Aqueous adsorption of aniline, phenol, and their substitutes by multi-walled carbon nanotubes. Environmental Science & Technology. ,vol. 42, pp. 7931- 7936 ,(2008) , 10.1021/ES801463V
Xiaoming Sun, Zhuang Liu, Kevin Welsher, Joshua Tucker Robinson, Andrew Goodwin, Sasa Zaric, Hongjie Dai, None, Nano-Graphene Oxide for Cellular Imaging and Drug Delivery. Nano Research. ,vol. 1, pp. 203- 212 ,(2008) , 10.1007/S12274-008-8021-8
Amedea B. Seabra, Amauri J. Paula, Renata de Lima, Oswaldo L. Alves, Nelson Durán, Nanotoxicity of graphene and graphene oxide. Chemical Research in Toxicology. ,vol. 27, pp. 159- 168 ,(2014) , 10.1021/TX400385X
Xiujuan Fan, Guozheng Jiao, Lei Gao, Pengfei Jin, Xin Li, The preparation and drug delivery of a graphene–carbon nanotube–Fe3O4 nanoparticle hybrid Journal of Materials Chemistry B. ,vol. 1, pp. 2658- 2664 ,(2013) , 10.1039/C3TB00493G
R. S. Swathi, K. L. Sebastian, Resonance energy transfer from a dye molecule to graphene Journal of Chemical Physics. ,vol. 129, pp. 054703- 054703 ,(2008) , 10.1063/1.2956498
Amara L. Holder, Regine Goth-Goldstein, Donald Lucas, Catherine P. Koshland, Particle-Induced Artifacts in the MTT and LDH Viability Assays Chemical Research in Toxicology. ,vol. 25, pp. 1885- 1892 ,(2012) , 10.1021/TX3001708
Alberto Bianco, Graphene: safe or toxic? The two faces of the medal. Angewandte Chemie. ,vol. 52, pp. 4986- 4997 ,(2013) , 10.1002/ANIE.201209099
Yanli Chang, Sheng-Tao Yang, Jia-Hui Liu, Erya Dong, Yanwen Wang, Aoneng Cao, Yuanfang Liu, Haifang Wang, In vitro toxicity evaluation of graphene oxide on A549 cells Toxicology Letters. ,vol. 200, pp. 201- 210 ,(2011) , 10.1016/J.TOXLET.2010.11.016