Nano-biointeractions of PEGylated and bare reduced graphene oxide on lung alveolar epithelial cells: A comparative in vitro study.

摘要: Graphene and its derivatives have garnered significant scientific interest potential use in nano-electronics as well biomedicine. However the undesirable biological consequence, especially upon inhalation of particle, requires further investigations. This study aimed to elucidate nano-biointeractions PEGylated reduced graphene oxide (PrGO) (rGO) with that lung alveolar epithelial cells (A549). Both nanomaterials showed dose dependent decrease cell viability alteration morphology after 24h. Upon intracellular uptake PrGO, it elicited oxidative stress mediated apoptosis by inducing ROS, loss mitochondrial membrane (MMP) inflammatory response NF-κB activation. Conversely, rGO was found scavenge ROS efficiently except at high It prompted MMP. adhere membrane, where is assumed bind surface Toll like receptors (TLRs) thereby activating response. All these events culminated an increase A549 24h exposure. also noticed both did not initiate lysosomal pathway but instead activated mitochondria apoptosis. highlights possible adverse toxic effect PrGO persistence particles lungs. Further research required comprehend so advance biomedical application safety.