Changes in the metabolism of modified and unmodified low-density lipoproteins during the maturation of cultured blood monocyte-macrophages from normal and homozygous familial hypercholesterolaemic subjects.

摘要: Blood monocyte-macrophages from homozygous familial hypercholesterolaemic (FH) patients showed the same pattern of morphological and biochemical changes during maturation in vitro as cells normal subjects. The protein content both types cell increased eightfold first two weeks culture while ratio free cholesterol to remained constant. specific activities lysosomal enzymes between days 2 5 again 7 12. activity 5′-nucleotidase rapidly then fell. Degradation low-density lipoprotein (LDL) by FH was not saturable and, when expressed per mg protein, gradually declined matured. Normal maintained medium containing whole serum degraded LDL an additional, process that was, at all times, decreased if were preincubated with a high concentration after preincubation lipoprotein-deficient serum. Saturable degradation LDL, DNA, highest fifth twelfth culture, which period greatest growth. results suggest incubated 20% receptors obtain required for their growth, but lack these did prevent development. Saturable acetylated or complexed dextran sulphate, each lower than unmodified 9. This increase corresponded development phagocytic properties cells, indicated release incubation opsonized zymosan.