NMR triple-quantum filtered relaxation analysis of 17O-water in insulin solutions: an insight into the aggregation of insulin and the properties of its bound water.
作者： Allan M. Torres , Stuart M. Grieve , Philip W. Kuchel
DOI: 10.1016/S0301-4622(97)00128-2
关键词: Insulin 、 Nuclear magnetic resonance spectroscopy 、 Random hexamer 、 Monomer 、 Crystallography 、 Analytical chemistry 、 Molecule 、 Properties of water 、 Chemistry 、 Bound water 、 Relaxation (NMR)
摘要: Abstract Transverse triple-quantum filtered NMR spectroscopy (TTQF) of 17 O-water was used to study the properties water in insulin solutions at different Zn 2+ concentrations and pH values. It established that strongly bound molecules are already present Zn-free insulin. On assumption effective correlation time a molecule, τ sb , is 10 ns, apparent number ∼3 4 per monomer. Addition equivalent ∼2 g-atoms hexamer did not produce substantial increases overall TTQF signal intensity fraction water. The dramatic enhancement signals observed for samples with /hexamer ratio greater than ∼2:1 could be attributed increase water, due formation higher-order oligomers protein.
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