Expression of Class 2 mannosidase and Class III mannosidase in lower eukaryotic cells
作者： Stephen R. Hamilton
DOI:
关键词: Biochemistry 、 Substrate specificity 、 Class iii 、 Eukaryote 、 Glycoprotein 、 Secretory pathway 、 Chemistry 、 Glycosidic bond 、 Mannosidase 、 Hydrolysis
摘要: A method for producing human-like glycoproteins by expressing a Class 2 α-mannosidase having substrate specificity Manα1,3 and Manα1,6 glycosidic linkages in lower eukaryote is disclosed. Hydrolysis of these on oligosaccharides produces substrates further N-glycan processing the secretory pathway.
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