Candida rugosa lipase immobilization on various chemically modified Chromium terephthalate MIL-101

作者: Atefeh Zare , Abdol-Khalegh Bordbar , Faranak Jafarian , Shahram Tangestaninejad

DOI: 10.1016/J.MOLLIQ.2018.01.097

关键词: Thermal stabilityGlutaraldehydeMetal-organic frameworkCandida rugosaFourier transform infrared spectroscopyChromiumNuclear chemistryLipaseSpecific activityChemistry

摘要: Abstract The paper seeks to immobilize Candida rugosa lipase (CRL) on Chromium terephthalate MIL-101 (MIL-101(Cr)) and its three chemically modified forms: amino MIL-101(Cr) (NH2-MIL), trichlorotriazine (TCT@NH2-MIL) glutaraldehyde (Glu@NH2-MIL). synthesis process of these metal organic frameworks, CRL immobilization the morphology supports were verified using FTIR, PXRD, BET FE-SEM techniques. enzyme loading specific activity at different initial concentration for all measured obtained results compared. highest any given point in common range belongs CRL@Glu@NH2-MIL. While immobilized CRLs show no significant drop residual after pH stress, thermal stability is just substantially improved CRL@TCT@NH2-MIL About 80–90% enzymatic retained 35 days indicating a storage CRLs.

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