Consensus Comparative Analysis of Human Embryonic Stem Cell-Derived Cardiomyocytes

摘要: Global transcriptional analyses have been performed with human embryonic stem cells (hESC) derived cardiomyocytes (CMs) to identify molecules and pathways important for CM differentiation, but variations in culture profiling conditions led greatly divergent results among different studies. Consensus investigation genes gene sets enriched multiple studies is revealing differential expression intrinsic differentiation independent of the above variables, reliable methods conducting such comparison are lacking. We examined between hESC hESC-CMs from microarray For single analysis, we identified that were expressed at increased levels seven datasets which not previously highlighted. set developed a new algorithm, consensus comparative analysis (CSSCMP), capable evaluating enrichment heterogeneous data sources. Based on both theoretical experimental validation, CSSCMP more efficient less susceptible than traditional methods. applied hESC-CM revealed novel (e.g., glucocorticoid stimulus), also might mediate this response. Our provide molecular information differentiation. Data Matlab codes can be downloaded S1 Data.