Molecular signature of cardiomyocyte clusters derived from human embryonic stem cells.

摘要: Human embryonic stem cells (hESCs) can differentiate in vitro into spontaneously contracting cardiomyocytes (CMs). These may prove extremely useful for various applications basic research, drug discovery, and regenerative medicine. To fully use the potential of cells, they need to be extensively characterized, regulatory mechanisms that control hESC differentiation toward cardiac lineage better defined. In this study, we used microarrays analyze, first time, global gene expression profile isolated hESC-derived CM clusters. By comparing clusters with undifferentiated hESCs using stringent selection criteria, identified 530 upregulated 40 downregulated genes further characterize family clusters, were classified according their Gene Ontology annotation. The results indicate display high similarities, on a molecular level, human heart tissue. Moreover, genes, created protein interaction maps revealed topological characteristics. We also searched cellular pathways among eight significantly pathways. Real-time quantitative polymerase chain reaction immunohistochemical analysis confirmed subset by microarrays. Taken together, presented here provide signature our understanding biological processes are active these cells.