Experimental infection of white spot baculovirus in some cultured and wild decapods in Taiwan

摘要: Techniques for the detection of white spot baculovirus virus (WSBV) by polymerase chain reaction are well established. In this study, two primer sets designed from an isolate WSBV Penaeus monodon, PmNOB III, were used to detect infection in cultured and wild decapods Taiwan. positive cases found all four major marine shrimp, P. japonicus, penicillatus Metapenaeus ensis. Wild semisulcatus was also be naturally infected WSBV. On other hand, no occurring have yet been shrimp Exopalaemen orientalis (from a milkfish culture farm), Trachypenaeus curvirostris, M. ensis coast Taiwan), Macrobrachium sp. Procambarus clarkii rivers Taiwan). Furthermore, neither crabs, Calappa lophos, Portunus sanguinolentus, Charybdis granulata C. feriata, nor lobsters Panulirus ornatus, versicolor, longipes penicillatus, collected Taiwan showed any evidence being When captured specimens these artificially feeding them with tissues severely III mortality reached moderate high levels at 18 days post infection. Using PCR analysis, DNA could detected moribund during experimental period survivors on final day experiment. The mortalities crabs lobsters, however, not significantly different control groups. Nevertheless, detectable specimens. thus shown wide host range exhibit infectivity various investigated present study.