Two approaches to form antibacterial surface: Doping with bactericidal element and drug loading

摘要: Abstract Two approaches (surface doping with bactericidal element and loading of antibiotic into specially formed surface microcontainers) to the fabrication antibacterial yet biocompatible bioactive surfaces are described. A network structure square-shaped blind pores 2.6 ± 0.6 × 10 −3  mm 3 for drug was obtained by selective laser sintering (SLS). The SLS-fabricated samples were loaded 0.03, 0.3, 2.4, 4 mg/cm 2 co-amoxiclav (amoxicillin clavulanic acid). Ag-doped TiCaPCON films 0.4, 1.2, 4.0 at.% Ag co-sputtering composite TiC 0.5 -Ca (PO 4 ) metallic targets. SLS-prepared cross-sectional morphology TiCaPCON-Ag studied scanning electron microscopy. through-thickness distribution in glow discharge optical emission spectroscopy. kinetics ion release normal saline solution using inductively coupled plasma mass spectrometry. Bacterial activity evaluated against S. epidermidis , aureus K. pneum. ozaenae agar diffusion test photometric method controlling variation density bacterial suspension over time. Cytocompatibility observed vitro chondrocytic MC3T3-E1 osteoblastic cells. viability proliferation cells determined MTS assay PicoGreen tests, respectively. alkaline phosphatase (ALP) also studied. results showed that moderate bacteriostatic effect is mainly manifested change colony densities bacteria suspensions. In contrast, a very rapid initial resulting strong just from start as long several days. ALP tests demonstrated it possible achieve pronounced compatible or even higher than control sample (standard disk amoxicillin/clavulanic acid mixture (30 μg)) without compromising material biocompatibility bioactivity.