Additive concentration effects on enantioselective separations in supercritical fluid chromatography

摘要: Polar additive concentration effects in supercritical fluid chromatography were studied on chiral stationary phases having either a macrocyclic glycopeptide or derivatized polysaccharide as the selector. Two basic additives, isopropylamine and triethylamine, incorporated into methanol modifier at various concentrations retention, selectivity, resolution monitored. Many of analytes failed to elute from phase absence an most noticeable effect increasing was significant decrease retention. On additives had little but they did foster improvements peak shape resolution.