Detection of copy number changes at multiple loci in DNA prepared from formalin-fixed, paraffin-embedded tissue by multiplex ligation-dependent probe amplification.
作者： Minoru Takata
DOI: 10.1007/978-1-59745-188-8_7
关键词: Multiplex ligation-dependent probe amplification 、 genomic DNA 、 DNA 、 Chemistry 、 Formalin fixed paraffin embedded 、 Pcr cloning 、 Molecular biology 、 Nucleic acid 、 Multiplex 、 Hybridization probe
摘要: With the increasing knowledge on genetic alterations associated with various cancers, molecular analysis of these formalin-fixed paraffin-embedded tissue is importance. Multiplex ligation-dependent probe amplification (MLPA) a novel technique to measure copy number up 45 nucleic acid sequences in single reaction. This method relies sequence-specific hybridization genomic DNA followed by multiplex-PCR hybridized probe, and semi-quantitative resulting PCR products. easy perform; requiring as little 50 ng extracted from formalin-fixed, (FFPE) tissue.
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