Multiplex ligation-dependent probe amplification for the detection of chromosomal gains and losses in formalin-fixed tissue.
作者： Marcory C van Dijk , Paul D Rombout , Sandra H Boots-Sprenger , Huub Straatman , Monique R Bernsen
DOI: 10.1097/01.PAS.0000146701.98954.47
关键词: Multiplex ligation-dependent probe amplification 、 Multiplex 、 Comparative genomic hybridization 、 Molecular analysis 、 Research setting 、 Aneuploidy 、 Formalin fixed 、 DNA sequencing 、 Molecular biology 、 Biology
摘要: Molecular analysis on formalin-fixed paraffin-embedded tissue is of increasing importance in diagnostic histopathology and tumor research. Multiplex ligation-dependent probe amplification (MLPA) a technique that can be used for detection copy number alterations up to 45 different DNA sequences one experiment. It performed partially degraded DNA, which makes this very suitable lesions. We tested the reliability MLPA by analyzing isolated from melanomas were previously characterized comparative genomic hybridization (CGH), additionally applicability was 29 routinely processed melanocytic appears reliable efficient method evaluate changes as 86% loci revealed concordant CGH results. Discordance mainly involved detected not probably due combination lower resolution occasionally false positive For application setting, probes specific region interest should prevent In research setting well fast screen large numbers lesions gains losses.
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