Alterations of 9p21 analysed by FISH and MLPA distinguish atypical spitzoid melanocytic tumours from conventional Spitz's nevi but do not predict their biological behaviour.

摘要: Cesinaro A M, Schirosi L, Bettelli S, Migaldi M & Maiorana (2010) Histopathology 57, 515–527 Alterations of 9p21 analysed by FISH and MLPA distinguish atypical spitzoid melanocytic tumours from conventional Spitz’s nevi but do not predict their biological behaviour Aim:  The aim this study was to investigate whether status influence the prognosis tumours, peculiar lesions whose behaviour cannot be predicted histopathological criteria alone. Methods results:  Twenty-eight 12 one congenital nevus were studied fluorescent in-situ hybridization (FISH) multiple ligation-dependent probe amplification (MLPA) for presence deletion. 28 patients aged 3–56 years (mean 32, median 35), follow-up ranged between 4 156 months 51, 48). Eight (28.5%) experienced lymph node metastasis (three cases with macrometastasis five micrometastasis). Of those macrometastasis, two are alive after 159 26 months, whereas a third developed widespread metastases died 26 months. All other alive. Statistically, thickness (P = 0.01) diameter (P = 0.009) significantly correlated metastasis. Deletion analysis observed in eight (28.5%), demonstrated alterations 9p21, particularly deletion CDKN2A, same lesions, all nevi, except one, unaltered (P < 0.0001). 9p21/CDKN2A did correlate metastasis. Conclusion:  at locus more frequent than behaviour.