Detection of HER2 amplification in breast carcinomas: comparison of Multiplex Ligation-dependent Probe Amplification (MLPA) and Fluorescence In Situ Hybridization (FISH) combined with automated spot counting.
作者： Rens L. H. P. M. van Hezik , Adriaan J. C. van den Brule , Toine C. J. M. van der Aa , Elna Moerland , Mike W. P. M. van Beek
DOI: 10.1155/2006/741586
关键词: Multiplex ligation-dependent probe amplification 、 Fish <Actinopterygii> 、 Gene duplication 、 Nucleic acid amplification technique 、 Biology 、 HER2 Amplification 、 Fluorescence in situ hybridization 、 HercepTest 、 Multiplex 、 Molecular biology
摘要: In this study the detection of HER2 gene amplification was evaluated using Fluorescence Situ Hybridization (FISH; PathVysion) in comparison with Multiplex Ligation-dependent Probe Amplification (MLPA), a PCR based technique. These two methods were on series 46 formalin fixed paraffin embedded breast carcinomas, previously tested for protein overexpression by HercepTest (grouped into Hercep 1+, 2+ and 3+). (ratio ≥ 2.0) FISH found 9/10, 10/30 0/6 IHC 3+, 1+/0 cases, respectively. Digitalized automated spot counting performed recently developed CW4000 CytoFISH software 100% concordant manual scoring. Using MLPA 18/46 samples showed clear amplification. Comparing same results as IHC. All but one positive cases (18/19) confirmed presence The overall concordance Her2 98% (45/46). Furthermore, both level equivocal correlated well between methods. conclusion, is reliable reproducible technique can be used an either alternative or additional test to determine status carcinomas.
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