Chromosomal copy number analysis in melanoma diagnostics.

摘要: The majority of melanocytic neoplasms can be correctly diagnosed using routine histopathologic analysis. However, a significant minority tumors have ambiguous attributes that overlap between nevi and melanoma. Ancillary tests assist in distinguishing potentially lethal melanomas from benign with atypical features are available, but still need refining.Most chromosomal copy number aberrations, frequently involving multiple chromosomes. With rare exceptions, such anomalies not found nevi. This difference formed the basis to develop assays help distinguish melanoma by fluorescence situ hybridization (FISH) comparative genomic (CGH). FISH detect changes limited loci within individual cells. By contrast, CGH assesses across entire genome, typically is performed on bulk cell populations so cells or subpopulations go undetected. Both been used provide information histopathologically pathologists make correct diagnoses.