Analysis of DNA copy number aberrations by multiple ligation-dependent probe amplification on 50 intestinal type gastric cancers.

摘要: Background and Objectives The molecular genetic alterations leading to gastric malignancy are largely unknown. This study aimed unravel the genomic DNA copy number aberrations (CNAs) profile during tumorigenesis. Methods In this study, we performed profiling in a set of 50 intestinal type carcinomas by PCR-based relative quantification method, multiple ligation-dependent probe amplification (MLPA) with 112 cancer-related gene loci selected throughout each human chromosome as probes MLPA assay. Results Numerous chromosomal CNAs, including gains 3p22, 4q25, 8q24, 11p13, 20q13, losses 1p36 9p21, were identified assay recurrent CNAs cancer. Moreover, found median numbers gains, losses, total significantly higher lymph node metastasis positive patients than cases without metastasis. And gain 11p13 9p21.3, 11q13.3, 17q25.3, 22q11.23 associated (P < 0.05). Finally, two major groups, G1 + 2 large G3 + 4 small can be successfully distinguished hierarchical cluster analysis. Conclusions Our results proved is reliable efficient method evaluate changes cancers. J. Surg. Oncol. 2011; 103:124–132. © 2010 Wiley-Liss, Inc.