Screening of DNA copy-number aberrations in gastric cancer cell lines by array-based comparative genomic hybridization

摘要: We performed genome-wide screening for deoxyribonucleic acid copy-number aberrations in 31 gastric cancer (GC) cell lines by using custom-made comparative genomic hybridization (CGH)-array. Copy-number gains were frequently detected at 1q, 3q, 5p, 7p, 7q, 8q, 11q, 17q, 20p, 20q, Xp and Xq, losses 3p, 4p, 4q, 8p, 9p, 18p 18q. With respect to histological subtypes, 1p, 16p, 20q 22q, 10p, 10q 18q significantly frequent derived from tumors of the well-differentiated type, whereas Xq undifferentiated type. Homozygous deletions seen five loci, high-level amplifications 15 GC lines; these had occurred 24 including segment containing CDK6 (7q21.2). Amplification that gene never been reported before. Immunohistochemical studies showed increased levels protein 54 292 primary samples we examined (18.5%). Cytoplasmic localization CDK6, as well over-expression, was more than tumors. Nuclear expression early stage advanced tumors, suggesting nuclear is likely be a prognostic factor GC. Taken together, our data indicate might involved pathogenesis and, generally, CGH-arrays have powerful potential identifying novel cancer-related genetic changes variety