Improved resolution by mounting of tissue sections for laser microdissection.

摘要: Isolated nucleic acids and proteins are the basic materials used for most current molecular techniques. To obtain reliable results, it is important that this starting material derived from a pure cell population. Two laser assisted microdissection methods to separate specific cells of interest infiltrating surrounding other cells. In capture microdissection, thermoplastic membrane placed above tissue attached after melting foil by low energy near infrared pulse. The remain embedded in film collected removal section.1 second method known as microbeam (LMM) uses pulsed ultraviolet (UV) an optical knife cut out target Separated transferred reaction tube needle, pressure catapulting, or gravity.2 advantage LMM unwanted adjacent can be easily photoablated UV beam. availability devices has greatly facilitated isolation populations even made possible identify mutations single cells.3,4 However, all use sections without coverslip. Most light passing through scattered, resulting obscured cellular detail poor microscopic image. This limited hampers recognition at histological level.5,6 overcome limitation, several approaches have been tried so far. mounted native suggested solution Bohm et al.7 Using technique, on subsequently upside down glass slide. approach does result improved morphology, although quite labourious, morphological quality decreases during microdissection. Alternatively, immunohistochemical detection situ hybridisation help.8,9 many cases, however, antibodies probes discriminate between may not available. For example, capability distinguish premalignant malignant relies characteristics, thus warranting optimal resolution. “The cells” Therefore, we developed mounting solves refraction problem results excellent morphology purposes.