Quantitative LAMMA analysis of biological specimens I. Standards. II. Isotope labeling

摘要: Besides a remarkably high sensitivity for light elements, laser microprobe mass spectroscopy offers two main advantages analysis: 1) the possibility to obtain data of relatively precision and 2) ability discriminate isotopes. This opens use cold isotopes as atomic labels study cellular or subcellular kinetics ions labelled organic compounds. In this photoreceptor cells were with 44Ca2+ prior LAMMA analysis monitor Ca2+ uptake and/or accumulation from extracellular sources into intracellular compartments such various pigment granula present in these photoreceptors. As other techniques also requires internal standards quantitative work. For purpose thin films inorganic material shown be useful when deposited (in vacuum) directly onto specimen analyzed. Some metals dielectrica have been tested their possible suitability standardisation procedures. Even though like Pt, Ag, Au, Al are excellent film production, they seem less because threshold induced perforation ion production is rather different that material. However, deposits some dielectric materials MgF2 turned out more suitable serve an standard plastic embedded biological analysis.