Rapid freezing of deep cerebral structures for electron microscopy
作者： A. van Harreveld , Eva Fifkova
关键词: Electron micrographs 、 Electron microscope 、 Biomedical engineering 、 Anatomy 、 Layer (electronics) 、 Head (vessel) 、 Materials science 、 Agricultural and Biological Sciences (miscellaneous)
摘要: A method is described for the investigation of deep cerebral structures by freeze-substitution. The head a mouse sectioned in guillotine-like apparatus. exposed surface subjected to Electron micrographs prepared from this material exhibit under layer sheared tissue, tissue comparable with that observed previously and cerebellar cortices freeze-substitution their natural surfaces. By varying plane which any structure brain can be made accessible
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