miR-138 overexpression is more powerful than hTERT knockdown to potentiate apigenin for apoptosis in neuroblastoma in vitro and in vivo
作者： Mrinmay Chakrabarti , Naren L. Banik , Swapan K. Ray
DOI: 10.1016/J.YEXCR.2013.02.025
关键词: Gene knockdown 、 Neuroblastoma 、 Telomerase reverse transcriptase 、 Small hairpin RNA 、 Cell growth 、 Telomerase 、 Cell culture 、 Cancer research 、 Biology 、 Transfection
摘要: Decrease in expression of the tumor suppressor microRNA-138 (miR-138) correlates well with an increase telomerase activity many human cancers. The ability almost all cancer cells to grow indefinitely is dependent on presence activity. catalytic component reverse transcriptase (hTERT) regulates most cancers including malignant neuroblastoma. We observed indirect miR-138 after transfection hTERT short hairpin RNA (shRNA) plasmid neuroblastoma SK-N-DZ and SK-N-BE2 cell lines. Transfection shRNA followed by treatment flavonoid apigenin (APG) further increased miR-138. Direct mimic was more powerful than potentiating efficacy APG for decreasing viability colony formation capability both Upregulation also effective down regulation enhancing induction apoptosis vitro vivo. delineated that occurred molecular components extrinsic intrinsic pathways In conclusion, these results demonstrate direct overexpression pro-apoptotic effect controlling growth culture animal models.
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