EWS Knockdown and Taxifolin Treatment Induced Differentiation and Removed DNA Methylation from p53 Promoter to Promote Expression of Puma and Noxa for Apoptosis in Ewing's Sarcoma.

摘要: Ewing’s sarcoma is a pediatric tumor that mainly occurs in soft tissues and bones. Malignant characteristics of are correlated with expression EWS oncogene. We achieved knockdown using plasmid vector encoding short hairpin RNA (shRNA) to increase anti-tumor mechanisms taxifolin (TFL), new flavonoid, human cells culture animal models. Immunofluorescence microscopy flow cytometric analysis showed high SK-N-MC RD-ES cell lines. shRNA plus TFL inhibited 80% viability caused the highest decreases at mRNA protein levels both Knockdown induced morphological features differentiation. more alterations molecular markers differentiation than either agent alone. migration inhibition survival, angiogenic invasive factors. was associated removal DNA methylation from p53 promoter, promoting p53, Puma, Noxa. amounts apoptosis activation extrinsic intrinsic pathways lines culture. also growth tumors models due inhibitors factors induction caspase-3 for apoptosis. Collectively, increased various