Epidermal Growth Factor Receptor Regulation of Ewing Sarcoma Cell Function.

摘要: Objective: Ewing sarcoma (ES) is a type of childhood cancer probably arising from stem mesenchymal or neural crest cells. The epidermal growth factor receptor (EGFR) acts as driver oncogene in many types solid tumors. However, its involvement ES remains poorly understood. Methods: Human SK-ES-1 and RD-ES cells were treated with EGF, the EGFR inhibitor tyrphostin (AG1478), phosphoinositide 3-kinase (PI3K) extracellular-regulated kinase (ERK)/mitogen-activated (MAPK) inhibitors. Cell proliferation survival, cycle, senescence analyzed. protein content possible targets manipulation was measured by Western blot. Results: survival increased EGF inhibited AG1478. also altered cell inducing arrest G1 increasing sub-G1 population, reduced polyploidy population senescent In addition, AG1478 levels phosphorylated AKT (p-AKT), ERK, p-ERK, cyclin D1, brain-derived neurotrophic (BDNF), while enhancing p53 levels. impaired inhibitors PI3K alone combined Conclusions: Our findings reveal novel aspects regulation provide early evidence for antitumor activities ES.