The phospholipase C inhibitor U73122 increases cytosolic calcium in MDCK cells by activating calcium influx and releasing stored calcium
作者： Chung-Ren Jan , Chin-Man Ho , Sheng-Nan Wu , Ching-Jiunn Tseng
DOI: 10.1016/S0024-3205(98)00346-4
关键词: Endoplasmic reticulum 、 Cell biology 、 Chemistry 、 Bradykinin 、 Calcium in biology 、 Thapsigargin 、 Plasma membrane Ca2+ ATPase 、 Mitochondrion 、 Calcium 、 Phospholipase C
摘要: The effects of the phospholipase C (PLC) inhibitor U73122 on intracellular calcium levels ([Ca2+]i) were studied in MDCK cells. elevated [Ca2+]i dose-dependently. Ca2+ influx contributed to 75% 20 microM U73122-induced signals. pretreatment abolished transients evoked by ATP and bradykinin, suggesting that inhibited PLC. signals among individual cells varied considerably. internal source for response was endoplasmic reticulum (ER) since thapsigargin. depletion ER store triggered a La3+-sensitive capacitative entry. Independently release Ca2 entry, directly through La3+-insensitive pathway. augmented carbonylcyanide m-chlorophynylhydrozone (CCCP), but not Na+ removal, implicating mitochondria significantly buffering signal, efflux via Na+/Ca2+ exchange insignificant.
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